Affordable Access

Crystallographic studies on a family B DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1.

Authors
  • Hashimoto, H
  • Matsumoto, T
  • Nishioka, M
  • Yuasa, T
  • Takeuchi, S
  • Inoue, T
  • Fujiwara, S
  • Takagi, M
  • Imanaka, T
  • Kai, Y
Type
Published Article
Journal
Journal of biochemistry
Publication Date
Jun 01, 1999
Volume
125
Issue
6
Pages
983–986
Identifiers
PMID: 10348896
Source
Medline
License
Unknown

Abstract

A hyperthermostable family B DNA polymerase from the hyperthermophilic archaeon, Pyrococcus kodakaraensis strain KOD1, has been crystallized by the hanging-drop vapor diffusion method at 293 K with 2-methyl-2,4-pentanediol as the precipitant. The diffraction pattern of a crystal extends to 3.0 A resolution, and two full sets of 3.0 A resolution diffraction data for native crystals were successfully collected at 290 K and 100 K upon exposure to synchrotron radiation at KEK-PF, Japan. The crystals belong to the space group, P212121, with unit-cell dimensions of a = 112.8, b = 115.4, and c = 75.4 A at 290 K, and a = 111.9, b = 112.4, and c = 73.9 at 100 K. Structural analysis by means of the multiple isomorphous replacement method is now in progress.

Report this publication

Statistics

Seen <100 times