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Crystallization and preliminary X-ray crystallographic analysis of the plant defensin NaD1.

Authors
  • Lay, Fung T1
  • Mills, Grant D
  • Hulett, Mark D
  • Kvansakul, Marc
  • 1 Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia. , (Australia)
Type
Published Article
Journal
Acta Crystallographica Section F Structural Biology and Crystallization Communications
Publisher
International Union of Crystallography
Publication Date
Jan 01, 2012
Volume
68
Issue
Pt 1
Pages
85–88
Identifiers
DOI: 10.1107/S1744309111049530
PMID: 22232180
Source
Medline
License
Unknown

Abstract

Plant defensins are small (~5 kDa) basic cysteine-rich proteins that are being explored in important agricultural crops for their ability to confer enhanced disease resistance against fungal pathogens. NaD1, isolated from the flowers of the ornamental tobacco (Nicotiana alata), is a particularly well characterized antifungal defensin. Here, the crystallization and preliminary X-ray crystallographic analysis of NaD1 is reported. Crystals of NaD1 were crystallized using the sitting-drop vapour-diffusion method at 291 K. Data were collected from two crystal forms to 1.4 and 1.6 Å resolution, respectively. The crystals of form A belonged to the monoclinic space group P2(1), with unit-cell parameters a = 32.697, b = 32.685, c = 41.977 Å, α = 90, β = 100.828, γ = 90°, whereas crystals of form B belonged to the trigonal space group P3(2)21, with unit-cell parameters a = b = 33.091, c = 128.77 Å, α = β = 90, γ = 120°.

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