Affordable Access

Crystallization and preliminary X-ray analysis of Escherichia coli peptidyl-tRNA hydrolase.

Authors
  • Schmitt, E
  • Fromant, M
  • Plateau, P
  • Mechulam, Y
  • Blanquet, S
Type
Published Article
Journal
Proteins
Publication Date
May 01, 1997
Volume
28
Issue
1
Pages
135–136
Identifiers
PMID: 9144799
Source
Medline
License
Unknown

Abstract

Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a = 47.24 A, b = 63.59 A, and c = 62.57 A. They belong to space group P2(1)2(1)2(1) and diffract to better than 1.2 A resolution. The structure is being solved by multiple isomorphous replacement.

Report this publication

Statistics

Seen <100 times