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Crystallization and preliminary diffraction data of neurotoxin Ts-gamma from the venom of the scorpion Tityus serrulatus.

Authors
Type
Published Article
Journal
Acta crystallographica. Section D, Biological crystallography
Publication Date
Volume
54
Issue
Pt 6 Pt 2
Pages
1440–1441
Identifiers
PMID: 10089532
Source
Medline
License
Unknown

Abstract

Scorpion neurotoxin Ts-gamma was isolated from Tityus serrulatus venom and purified to apparent homogeneity by ion-exchange HPLC. Crystals of the toxin were grown using polyethylene glycol 6000 as precipitant and were found to belong to the monoclinic space group P21 with cell parameters a = 22.20, b = 36.90, c = 31.57 A, beta = 100.85 degrees. The crystals diffract beyond 1.73 A resolution at a synchrotron beamline, being notably stable during X-ray exposure. The structure has been solved by molecular replacement using the very high resolution structure of Sahara scorpion Androctonus australis Hector (PDB code 1AHO) as a search model.

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