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Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids.

Authors
  • Teixeira, Weslen Fabricio Pires1
  • De Oliveira, Márcio Leite2
  • de Faria Peres, Pedro Henrique2
  • Nagata, Walter Bertequini3
  • Santana, Bruna Nicoleti3
  • Oliveira, Bruno César Miranda3
  • Duarte, José Maurício Barbanti2
  • Cardoso, Tereza Cristina3
  • Lopes, Welber Daniel Zanetti4
  • Bresciani, Katia Denise Saraiva5
  • 1 Federal University of Goiás (UFG), Goiânia, Goiás, Brazil; São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEP: 16050-680, Araçatuba, Brazil. , (Brazil)
  • 2 São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil. , (Brazil)
  • 3 São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEP: 16050-680, Araçatuba, Brazil. , (Brazil)
  • 4 Federal University of Goiás (UFG), Goiânia, Goiás, Brazil. , (Brazil)
  • 5 São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEP: 16050-680, Araçatuba, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Parasitology international
Publication Date
Feb 01, 2021
Volume
80
Pages
102216–102216
Identifiers
DOI: 10.1016/j.parint.2020.102216
PMID: 33137502
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids. Copyright © 2020. Published by Elsevier B.V.

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