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Cryptide signaling: Amphiphilic peptide-induced exocytotic mechanisms in mast cells

Authors
  • Mukai, Hidehito
  • Kikuchi, Miharu
  • Fukuhara, Shigetomo
  • Kiso, Yoshiaki
  • Munekata, Eisuke
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Publication Date
Jan 01, 2008
Volume
375
Issue
1
Pages
22–26
Identifiers
DOI: 10.1016/j.bbrc.2008.07.081
Source
Elsevier
Keywords
License
Unknown

Abstract

Amphiphilic peptides with positive charges such as substance P (SP) and mastoparan (MP) are known to induce exocytosis in rat peritoneal mast cells. To elucidate whether and how intracellular Ca 2+ signaling is involved in the peptide-induced exocytosis, here we investigated the relationships between an increase in intracellular free Ca 2+ concentration ([Ca 2+] i) and exocytosis caused by SP and MP. SP and MP induced exocytosis coinciding with an initial rapid and transient [Ca 2+] i increase, but not with a sustained increase. These stimulations were abolished by pertussis toxin, indicating the involvement of the G i-family of G proteins in the peptide signaling. Moreover, the [Ca 2+] i increase was shown to accelerate and potentiate exocytosis, suggesting that the transient increase in [Ca 2+] i positively modified exocytotic secretion. However, it was indicated that the signal of [Ca 2+] i increase was not sufficient for the peptide-induced exocytosis, suggesting the participation of alternative mechanisms other than Ca 2+ signaling in the pathway.

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