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Cryopreservation of somatic embryos and embryonic axes of Camellia japonica L.

Authors
  • Janeiro, L V
  • Vieitez, A M
  • Ballester, A
Type
Published Article
Journal
Plant cell reports
Publication Date
May 01, 1996
Volume
15
Issue
9
Pages
699–703
Identifiers
DOI: 10.1007/BF00231928
PMID: 24178614
Source
Medline
License
Unknown

Abstract

Cryopreservation in liquid nitrogen was attempted with both somatic embryos and zygotic embryonic axes of the ornamental Camellia japonica L. Several protective measures were applied to somatic embryos (desiccation, chemical protectors, hardening by culture at low temperatures, encapsulation in alginate beads), but none allowed somatic embryos cultures to survive after 24 h in liquid nitrogen. Embryonic axes, however, were easily cryopreserved by means of the simplest technique: desiccation in a laminar flow hood and direct immersion in liquid nitrogen. Although the causes of the difference in cryopreservability between the two types of material are not known, one might be the difference between their degrees of differentiation and water content.

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