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Cryo-Electron Tomography Studies of Cell Systems

Authors
  • Kamyshinsky, R. A.1, 2
  • Chesnokov, Y. M.1, 2
  • Orekhov, A. S.1, 2
  • 1 National Research Center “Kurchatov Institute”, Moscow, 123182, Russia , Moscow (Russia)
  • 2 Shubnikov Institute of Crystallography, Federal Research Center “Crystallography and Photonics”, Russian Academy of Sciences, Moscow, 119333, Russia , Moscow (Russia)
Type
Published Article
Journal
Crystallography Reports
Publisher
Pleiades Publishing
Publication Date
Sep 01, 2020
Volume
65
Issue
5
Pages
744–748
Identifiers
DOI: 10.1134/S1063774520050090
Source
Springer Nature
License
Yellow

Abstract

AbstractCryo-electron tomography is a powerful tool for determining the three-dimensional structures of macromolecular complexes in their natural environment. The minimization of external impacts on the investigated objects, possibility of conducting in vitro and in cellulo experiments under close-to-native conditions, and high spatial resolution of the obtained three-dimensional reconstructions make cryo-electron tomography one of the most promising methods for studying a large class of objects in the fields of structural biology and visual proteomics. The main aspects of cryo-electron tomography are discussed as applied to studies of cell systems.

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