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Crosstalk between chromatin structure, cohesin activity and transcription

  • Maya-Miles, Douglas1
  • Andújar, Eloísa2
  • Pérez-Alegre, Mónica2
  • Murillo-Pineda, Marina1, 3
  • Barrientos-Moreno, Marta1
  • Cabello-Lobato, María J.1, 4
  • Gómez-Marín, Elena1
  • Morillo-Huesca, Macarena1
  • Prado, Félix1
  • 1 CSIC-University of Seville-University Pablo de Olavide, Department of Genome Biology, Andalusian Molecular Biology and Regenerative Medicine (CABIMER), Seville, Spain , Seville (Spain)
  • 2 CSIC-University of Seville-University Pablo de Olavide, Genomic Unit, Andalusian Molecular Biology and Regenerative Medicine Center (CABIMER), Seville, Spain , Seville (Spain)
  • 3 University of Oxford, Department of Biochemistry, Oxford, UK , Oxford (United Kingdom)
  • 4 University of Manchester, Division of Cancer Sciences, Manchester Cancer Research Center, Manchester, UK , Manchester (United Kingdom)
Published Article
Epigenetics & Chromatin
Springer (Biomed Central Ltd.)
Publication Date
Jul 22, 2019
DOI: 10.1186/s13072-019-0293-6
Springer Nature


BackgroundA complex interplay between chromatin and topological machineries is critical for genome architecture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its multiple roles in DNA metabolism.ResultsWe have used genome-wide analyses to address how cohesins and chromatin structure impact each other in yeast. Cohesin inactivation in scc1-73 mutants during the S and G2 phases causes specific changes in chromatin structure that preferentially take place at promoters; these changes include a significant increase in the occupancy of the − 1 and + 1 nucleosomes. In addition, cohesins play a major role in transcription regulation that is associated with specific promoter chromatin architecture. In scc1-73 cells, downregulated genes are enriched in promoters with short or no nucleosome-free region (NFR) and a fragile “nucleosome − 1/RSC complex” particle. These results, together with a preferential increase in the occupancy of nucleosome − 1 of these genes, suggest that cohesins promote transcription activation by helping RSC to form the NFR. In sharp contrast, the scc1-73 upregulated genes are enriched in promoters with an “open” chromatin structure and are mostly at cohesin-enriched regions, suggesting that a local accumulation of cohesins might help to inhibit transcription. On the other hand, a dramatic loss of chromatin integrity by histone depletion during DNA replication has a moderate effect on the accumulation and distribution of cohesin peaks along the genome.ConclusionsOur analyses of the interplay between chromatin integrity and cohesin activity suggest that cohesins play a major role in transcription regulation, which is associated with specific chromatin architecture and cohesin-mediated nucleosome alterations of the regulated promoters. In contrast, chromatin integrity plays only a minor role in the binding and distribution of cohesins.

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