Affordable Access

deepdyve-link
Publisher Website

High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination

Authors
  • Tan, Yann-Chong
  • Blum, Lisa K.
  • Kongpachith, Sarah
  • Ju, Chia-Hsin
  • Cai, Xiaoyong
  • Lindstrom, Tamsin M.
  • Sokolove, Jeremy
  • Robinson, William H.1, 2, 3, 4, 5, 6, 7
  • 1 Division of Immunology and Rheumatology
  • 2 Stanford University
  • 3 VA Palo Alto Health Care System
  • 4 3801 Miranda Ave
  • 5 Palo Alto
  • 6 Stanford Immunology Program
  • 7 Stanford University School of Medicine
Type
Published Article
Journal
Clinical Immunology
Publisher
Elsevier BV
Publication Date
Jan 01, 2014
Accepted Date
Dec 30, 2013
Volume
151
Issue
1
Pages
55–65
Identifiers
DOI: 10.1016/j.clim.2013.12.008
Source
Elsevier
Keywords
License
Unknown

Abstract

•Deep-sequencing of plasmablast antibody repertoires to influenza vaccination•Single-cell barcoding enables recovery of natively paired heavy- and light-chains.•Identification of clonal families of antibodies with shared rearrangements•Antibodies from clonal populations are enriched for binding and neutralization.•Some antibodies induced by influenza vaccination exhibit original antigenic sin.

Report this publication

Statistics

Seen <100 times