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Crop-Zone Weed Mycobiomes of the South-Western Australian Grain Belt

Authors
  • Michael, Pippa J.1
  • Jones, Darcy1
  • White, Nicole2
  • Hane, James K.1
  • Bunce, Michael2
  • Gibberd, Mark1
  • 1 Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Perth, WA , (Australia)
  • 2 TRENDLab, School of Molecular and Life Sciences, Curtin University, Perth, WA , (Australia)
Type
Published Article
Journal
Frontiers in Microbiology
Publisher
Frontiers Media SA
Publication Date
Nov 24, 2020
Volume
11
Identifiers
DOI: 10.3389/fmicb.2020.581592
PMID: 33324368
PMCID: PMC7721668
Source
PubMed Central
Keywords
License
Unknown

Abstract

In the absence of a primary crop host, secondary plant hosts may act as a reservoir for fungal plant pathogens of agricultural crops. Secondary hosts may potentially harbor heteroecious biotrophs (e.g., the stripe rust fungus Puccinia striiformis ) or other pathogens with broad host ranges. Agricultural grain production tends toward monoculture or a limited number of crop hosts over large regions, and local weeds are a major source of potential secondary hosts. In this study, the fungal phyllospheres of 12 weed species common in the agricultural regions of Western Australia (WA) were compared through high-throughput DNA sequencing. Amplicons of D2 and ITS were sequenced on an Illumina MiSeq system using previously published primers and BLAST outputs analyzed using MEGAN. A heatmap of cumulative presence–absence for fungal taxa was generated, and variance patterns were investigated using principal components analysis (PCA) and canonical correspondence analysis (CCA). We observed the presence of several major international crop pathogens, including basidiomycete rusts of the Puccinia spp., and ascomycete phytopathogens of the Leptosphaeria and Pyrenophora genera. Unrelated to crop production, several endemic pathogen species including those infecting Eucalyptus trees were also observed, which was consistent with local native flora. We also observed that differences in latitude or climate zones appeared to influence the geographic distributions of plant pathogenic species more than the presence of compatible host species, with the exception of Brassicaceae host family. There was an increased proportion of necrotrophic Ascomycete species in warmer and drier regions of central WA, compared to an increased proportion of biotrophic Basidiomycete species in cooler and wetter regions in southern WA.

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