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Critical Evaluation of Membrane Supports for Use in Quantitative Hybridizations

Authors
  • L. Raskin
  • W. C. Capman
  • M. D. Kane
  • B. E. Rittmann
  • D. A. Stahl
Publication Date
Jan 01, 1996
Source
PMC
Keywords
License
Unknown

Abstract

The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.

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