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Critical density for protein-protein monoconjugation on a polysaccharide matrix

Authors
  • Pillai, Shiv
  • Surolia, A.
  • Bachhawat, B.K.
Type
Published Article
Journal
BBA - Protein Structure
Publication Date
Jan 01, 1979
Volume
580
Issue
1
Pages
216–219
Identifiers
DOI: 10.1016/0005-2795(79)90212-5
Source
Elsevier
Keywords
License
Unknown

Abstract

The galactose binding toxin (RCA II) from Ricinus communis was affinity-immobilised at varying densities on a polysaccharide matrix and reacted with glutaraldehyde. The critical density below which inter-molecular cross-links were not formed was determined. At this density RCA II was monoconjugated to lysozyme. This approach could serve as a prototype for enzyme-lectin and enzyme-antipolysaccharide antibody monoconjugation.

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