Affordable Access

Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis.

Authors
  • 1
  • 1 Institute for Molecular Genetics and Animal Disease, Murdoch University, Australia. , (Australia)
Type
Published Article
Journal
Acta Tropica
0001-706X
Publisher
Elsevier
Publication Date
Volume
50
Issue
2
Pages
115–124
Identifiers
PMID: 1685867
Source
Medline
License
Unknown

Abstract

Isoenzyme electrophoresis using 13 enzyme systems was applied to 31 Australian and 7 Swiss isolates of Giardia of human, cat, cattle, dog, sheep and rat origin. The Portland (ATCC No. 30888) reference strain was also included. The 39 isolates were divided into 22 different zymodemes. These consisted of 19 zymodemes containing the P1 and Australian isolates and three zymodemes containing Swiss isolates only. Differences in enzyme profiles between zymodemes was measured by euclidean distance and it was found that Australian isolates of Giardia exhibited more variation than the Swiss isolates. Relationships between zymodemes determined by clustering analysis are discussed with particular reference to the zoonotic potential of Giardia.

Statistics

Seen <100 times