Criterion of the synchronization (CS) of cells division for S. typhi population is proposed. The criterion is based on the assumption of the normal distribution of cells with different generation time in the population after stressor (shock) action. CS is equal to the ratio of the dispersion of the generation time of cells in the population to the average generation time of the whole population and determined from the parameters of the mathematical model. The quantitative values of the parameters of the mathematical model were obtained by the minimization of error between the calculation and experimental data. CS was used for the evaluation and choice of the optimum stressor action in the synchronization of the division of S. typhi.