Affordable Access

deepdyve-link
Publisher Website

Creation of a gold nanoparticle based electrochemical assay for the detection of inhibitors of bacterial cytochrome bd oxidases.

Authors
  • Fournier, Eugénie1
  • Nikolaev, Anton1
  • Nasiri, Hamid R2
  • Hoeser, Jo3
  • Friedrich, Thorsten3
  • Hellwig, Petra4
  • Melin, Frederic5
  • 1 Laboratoire de Bioelectrochimie et Spectroscopie, UMR 7140, Chimie de la Matière Complexe, Université de Strasbourg, CNRS, Strasbourg, France. , (France)
  • 2 Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Straße 7, D-60438 Frankfurt am Main, Germany. , (Germany)
  • 3 Albert-Ludwigs-Universität Freiburg, Institut für Biochemie, Freiburg, Germany. , (Germany)
  • 4 Laboratoire de Bioelectrochimie et Spectroscopie, UMR 7140, Chimie de la Matière Complexe, Université de Strasbourg, CNRS, Strasbourg, France. Electronic address: [email protected] , (France)
  • 5 Laboratoire de Bioelectrochimie et Spectroscopie, UMR 7140, Chimie de la Matière Complexe, Université de Strasbourg, CNRS, Strasbourg, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Date
Oct 01, 2016
Volume
111
Pages
109–114
Identifiers
DOI: 10.1016/j.bioelechem.2016.06.001
PMID: 27314676
Source
Medline
Keywords
License
Unknown

Abstract

Cytochrome bd oxidases are membrane proteins expressed by bacteria including a number of pathogens, which make them an attractive target for the discovery of new antibiotics. An electrochemical assay is developed to study the activity of these proteins and inhibition by quinone binding site tool compounds. The setup relies on their immobilization at electrodes specifically modified with gold nanoparticles, which allows achieving a direct electron transfer to/from the heme cofactors of this large enzyme. After optimization of the protein coverages, the assay shows at pH7 a good reproducibility and readout stability over time, and it is thus suitable for further screening of small molecule collections.

Report this publication

Statistics

Seen <100 times