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CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.

Authors
  • 1
  • 1 Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, USA.
Type
Published Article
Journal
The Journal of Infectious Diseases
1537-6613
Publisher
Oxford University Press
Publication Date
Volume
205
Issue
7
Pages
1095–1099
Identifiers
DOI: 10.1093/infdis/jis032
PMID: 22357696
Source
Medline
License
Unknown

Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

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