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CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.

Authors
  • Shamay, Meir1
  • Hand, Nicholas
  • Lemas, M Victor
  • Koon, Henry B
  • Krown, Susan E
  • Wrangle, John
  • Desai, Prashant
  • Ramos, Juan Carlos
  • Ambinder, Richard F
  • 1 Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, USA.
Type
Published Article
Journal
The Journal of Infectious Diseases
Publisher
Oxford University Press
Publication Date
Apr 01, 2012
Volume
205
Issue
7
Pages
1095–1099
Identifiers
DOI: 10.1093/infdis/jis032
PMID: 22357696
Source
Medline
License
Unknown

Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

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