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COVID-19 Molecular Testing in Korea: Practical Essentials and Answers From Experts Based on Experiences of Emergency Use Authorization Assays

Authors
  • Sung, Heungsup
  • Roh, Kyoung Ho
  • Hong, Ki Ho
  • Seong, Moon-Woo
  • Ryoo, Namhee
  • Kim, Hyun Soo
  • Lee, Jaehyeon
  • Kim, So Yeon
  • Yoo, Sookwon
  • Kim, Mi-Na
  • Han, Myung Guk
  • Lee, Sang Won
  • Lee, Hyukmin
  • Yoo, Cheon Kwon
Type
Published Article
Journal
Annals of Laboratory Medicine
Publisher
Korean Society for Laboratory Medicine
Publication Date
Nov 01, 2020
Volume
40
Issue
6
Pages
439–447
Identifiers
DOI: 10.3343/alm.2020.40.6.439
PMID: 32539299
PMCID: PMC7295959
Source
PubMed Central
Keywords
License
Unknown

Abstract

Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early detection of COVID-19 and immediate isolation of infected patients from the naive population are important to prevent further pandemic spread of the infection. Real-time reverse transcription (RT)-PCR to detect SARS-CoV-2 RNA is currently the most reliable diagnostic method for confirming COVID-19 worldwide. Guidelines for clinical laboratories on the COVID-19 diagnosis have been recently published by Korean Society for Laboratory Medicine and the Korea Centers for Disease Control and Prevention. However, these formal guidelines do not address common practical laboratory issues related to COVID-19 real-time RT-PCR testing and their solutions. Therefore, this guideline is intended as a practical and technical supplement to the “Guidelines for Laboratory Diagnosis of COVID-19 in Korea”.

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