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Covalent immobilization of delipidated human serum albumin on poly(pyrrole-2-carboxylic) acid film for the impedimetric detection of perfluorooctanoic acid.

Authors
  • Moro, Giulia1
  • Bottari, Fabio2
  • Liberi, Stefano3
  • Covaceuszach, Sonia4
  • Cassetta, Alberto4
  • Angelini, Alessandro5
  • De Wael, Karolien2
  • Moretto, Ligia Maria3
  • 1 Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy; AXES Research Group, Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium; NANOlab Center of Excellence, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address: [email protected] , (Belgium)
  • 2 AXES Research Group, Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium; NANOlab Center of Excellence, Groenenborgerlaan 171, 2020 Antwerp, Belgium. , (Belgium)
  • 3 Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy. , (Italy)
  • 4 Istituto di Cristallografia - CNR, Trieste Outstation, Italy SS 14 km 163.5, Basovizza, Trieste, Italy. , (Italy)
  • 5 Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy; European Centre for Living Technology (ECLT), Ca' Bottacin, Dorsoduro 3911, Calle Crosera, 30123 Venice, Italy. , (Italy)
Type
Published Article
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Date
Apr 26, 2020
Volume
134
Pages
107540–107540
Identifiers
DOI: 10.1016/j.bioelechem.2020.107540
PMID: 32361666
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The immobilization of biomolecules at screen printed electrodes for biosensing applications is still an open challenge. To enrich the toolbox of bioelectrochemists, graphite screen printed electrodes (G-SPE) were modified with an electropolymerized film of pyrrole-2-carboxilic acid (Py-2-COOH), a pyrrole derivative rich in carboxylic acid functional groups. These functionalities are suitable for the covalent immobilization of biomolecular recognition layers. The electropolymerization was first optimized to obtain stable and conductive polymeric films, comparing two different electrolytes: sodium dodecyl sulphate (SDS) and sodium perchlorate. The G-SPE modified with Py-2-COOH in 0.1 M SDS solution showed the required properties and were further tested. A proof-of-concept study for the development of an impedimetric sensor for perfluorooctanoic acid (PFOA) was carried out using the delipidated human serum albumin (hSA) as bioreceptor. The data interpretation was supported by size exclusion chromatography and small-angle X-ray scattering (SEC-SAXS) analysis of the bioreceptor-target complex and the preliminary results suggest the possibility to further develop this biosensing strategy for toxicological and analytical studies. Copyright © 2020. Published by Elsevier B.V.

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