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Coupling Neuropeptide Levels to Structural Plasticity in Drosophila Clock Neurons.

Authors
  • Herrero, Anastasia1
  • Yoshii, Taishi2
  • Ispizua, Juan Ignacio1
  • Colque, Carina1
  • Veenstra, Jan A3
  • Muraro, Nara I1
  • Ceriani, María Fernanda4
  • 1 Laboratorio de Genética del Comportamiento, Fundación Instituto Leloir, IIB-BA CONICET, Av. Patricias Argentinas 435, 1405 Buenos Aires, Argentina. , (Argentina)
  • 2 Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. , (Japan)
  • 3 INCIA UMR 5287 CNRS, Université de Bordeaux, allée Geoffroy St Hillaire, CS 50023, 33 615 Pessac Cedex, France. , (France)
  • 4 Laboratorio de Genética del Comportamiento, Fundación Instituto Leloir, IIB-BA CONICET, Av. Patricias Argentinas 435, 1405 Buenos Aires, Argentina. Electronic address: [email protected] , (Argentina)
Type
Published Article
Journal
Current biology : CB
Publication Date
Aug 17, 2020
Volume
30
Issue
16
Identifiers
DOI: 10.1016/j.cub.2020.06.009
PMID: 32619484
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We have previously reported that pigment dispersing factor (PDF) neurons, which are essential in the control of rest-activity cycles in Drosophila, undergo circadian remodeling of their axonal projections, a phenomenon called circadian structural plasticity. Axonal arborizations display higher complexity during the day and become simpler at night, and this remodeling involves changes in the degree of connectivity. This phenomenon depends on the clock present within the ventrolateral neurons (LNvs) as well as in glia. In this work, we characterize in detail the contribution of the PDF neuropeptide to structural plasticity at different times across the day. Using diverse genetic strategies to temporally restrict its downregulation, we demonstrate that even subtle alterations to PDF cycling at the dorsal protocerebrum correlate with impaired remodeling, underscoring its relevance for the characteristic morning spread; PDF released from the small LNvs (sLNvs) and the large LNvs (lLNvs) contribute to the process. Moreover, forced depolarization recruits activity-dependent mechanisms to mediate growth only at night, overcoming the restriction imposed by the clock on membrane excitability. Interestingly, the active process of terminal remodeling requires PDF receptor (PDFR) signaling acting locally through the cyclic-nucleotide-gated channel ion channel subunit A (CNGA). Thus, clock-dependent PDF signaling shapes the connectivity of these essential clock neurons on daily basis. Copyright © 2020 Elsevier Inc. All rights reserved.

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