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Costimulatory Molecule CD28 Participates in the Process of Embryo Implantation in Mice

Authors
  • Liu, Shangjing1
  • He, Junlin1
  • Chen, Xuemei1
  • Ding, Yubin1
  • Geng, Yanqing1
  • Wu, Mengyun1
  • Liu, Xueqing1
  • Wang, Yingxiong1
  • 1 Chongqing Medical University, No. 1 Yixueyuan Rd, Yuzhong District, Chongqing, 400016, People’s Republic of China , Chongqing (China)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Jun 01, 2014
Volume
21
Issue
6
Pages
686–695
Identifiers
DOI: 10.1177/1933719113512537
Source
Springer Nature
Keywords
License
Yellow

Abstract

Embryo implantation is a complex process requiring reciprocal interactions between implantation-competent blastocysts and receptive uteri. Accumulating literatures have indicated that T cells are involved in this process. The first signal mediated by T-cell receptor/CD3 complex and the second signal delivered by costimulatory molecules are essential for the differentiation of T cell into an effector cell. Expression and function of CD28, an important costimulatory molecule, during early pregnancy in mice is still unclear. In the present study, we investigated the expression pattern of CD28 in mouse uterus during early pregnancy and pseudopregnancy by real-time quantitative polymerase chain reaction, Western blotting, in situ hybridization, and immunohistochemistry (IHC). We found that injection of the uterine horn with CD28 antisense oligodeoxynucleotides leads to a decreased number of implantation sites. The expression pattern of CD3 protein examined by IHC is similar to that of CD28. These findings suggest that CD28 participates in the process of embryo implantation in mice, which might play its role through delivering the second costimulatory signal.

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