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Cost-effective, species-specific microsatellite development for the endangered Dwarf Bulrush (Typha minima) using next-generation sequencing technology.

Authors
  • Csencsics, Daniela
  • Brodbeck, Sabine
  • Holderegger, Rolf
Type
Published Article
Journal
The Journal of heredity
Publication Date
Jan 01, 2010
Volume
101
Issue
6
Pages
789–793
Identifiers
DOI: 10.1093/jhered/esq069
PMID: 20562212
Source
Medline
License
Unknown

Abstract

The dwarf bulrush (Typha minima Funck ex Hoppe) is an endangered pioneer plant species of riparian flood plains. In Switzerland, only 3 natural populations remain, but reintroductions are planned. To identify suitable source populations for reintroductions, we developed 17 polymorphic microsatellite markers with perfect repeats using the 454 pyrosequencing technique and tested them on 20 individuals with low-cost M13 labeling. We detected 2 to 7 alleles per locus and found expected and observed heterozygosities of 0.05-0.76 and 0.07-1, respectively. The whole process was finished in less than 6 weeks and cost approximately USD 5000. Due to low costs and reduced expenditure of time, the use of next-generation sequencing techniques for microsatellite development represent a powerful tool for population genetic studies in nonmodel species, as we show in this first application of the approach to a plant species of conservation importance.

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