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[Correlation of qAnti-HBc with antiviral efficacy in children with chronic hepatitis B and exploration of its possible immune mechanism].

Authors
  • Zhou, Y Z1
  • Chang, Y N1
  • He, Y1
  • Wang, H M1
  • Peng, X R1
  • Chen, M2
  • Peng, M L2
  • Hu, P2
  • Ren, H2
  • Xu, H M1
  • 1 Department of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing 400014, China. , (China)
  • 2 Department of Infectious Diseases, the Second Affiliated Hospital of Chongqing Medical University, Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Chongqing 400010, China. , (China)
Type
Published Article
Journal
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Publication Date
Sep 20, 2021
Volume
29
Issue
9
Pages
837–843
Identifiers
DOI: 10.3760/cma.j.cn501113-20210804-00376
PMID: 34638201
Source
Medline
Keywords
Language
Chinese
License
Unknown

Abstract

目的: 比较行抗病毒治疗的HBeAg阳性慢性乙型肝炎(CHB)患儿无应答组和应答组间基线乙型肝炎核心抗体定量(qAnti-HBc)水平的差异,以探讨不同qAnti-HBc水平患儿外周血CD8(+)记忆T细胞亚群比例及其功能活性。 方法: 回顾性检测2018年6月至2020年12月于重庆医科大学附属儿童医院感染科就诊的85例HBeAg阳性CHB患儿基线qAnti-HBc水平。分析其中37例抗病毒治疗患儿基线qAnti-HBc水平与HBeAg血清学应答的关系。流式细胞检测59例患儿基线外周血CD8(+)记忆T细胞亚群比例及干扰素(IFN)-γ、肿瘤坏死因子(TNF)-α分泌水平,分析qAnti-HBc水平与CD8(+)记忆T细胞亚群比例及其功能活性间的关系。计数资料比较采用Pearson’s Chi-square检验,两组或多组间计量资料比较采用Mann-Whitney U检验或Kruskal-Wallis检验,连续性变量间的相关性采用Spearman秩相关分析。 结果: 在37例接受恩替卡韦(ETV,21/37)或聚乙二醇干扰素(Peg-IFN,16/37)治疗的患儿中,有18例发生HBeAg血清学转换(ETV组10/21,Peg-IFN组8/16),应答组患儿基线qAnti-HBc水平[4.71(4.64~4.81)log(10)IU/ml]显著高于无应答组[4.54(4.45~4.64)log(10)IU/ml,Z = -3.316,P = 0.001]。高qAnti-HBc组CD8(+)Tem、CD38(+)CD8(+)Tem、CD38(+)CD8(+)Temra细胞比例及CD8(+)T细胞分泌的IFN-γ、TNF-α水平显著高于低qAnti-HBc组(P < 0.05);ALT>1×正常值上限(ULN)组CD8(+)Tem、CD38(+)CD8(+)Tem、CD38(+)CD8(+)Temra细胞比例显著高于ALT≤1×ULN组(P<0.05),但两组CD8(+)T细胞分泌的IFN-γ、TNF-α水平差异无统计学意义(P>0.05)。Spearman相关分析显示,qAnti-HBc与CD8(+)Tem、CD38(+)CD8(+)Tem、CD38(+)CD8(+)Temra细胞比例及CD8(+)T细胞分泌的IFN-γ水平呈正相关(P<0.05);ALT仅与CD38(+)CD8(+)Tem、CD38(+)CD8(+)Temra细胞比例呈正相关(P<0.05)。 结论: 较高的基线qAnti-HBc水平与CHB儿童患者抗病毒治疗HBeAg血清学应答相关;qAnti-HBc较高的CHB患儿外周血效应CD8(+)T细胞表现出更强的表型及功能活化特点,此研究可能对CHB儿童抗病毒治疗疗效相关的潜在免疫机制作出一定阐释。.

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