Colorectal cancer is one of the most common cancers in the world. This study aimed to investigate the correlation between microRNA-107 (miR-107) expression level and the prognosis in colorectal cancer patients with its clinical significance. 80 cases of cancer tissues and 15 cases of adjacent cancer tissues were collected from colorectal cancer patients treated with surgery from February 2006 to January 2010. The expression of miR-107 was detected by real-time PCR. The correlation between miR-107 expression and clinic pathological factors and survival time of patients was statistically analyzed. The expression level of miR-107 in cancer tissues (0.0213 ± 0.0096) was significantly higher than that in adjacent tissues (0.0355 ± 0.0487). The expressions of miR-107 in patients with different TNM stages, Dukes stages, and lymph node metastasis rates were significantly different (P < 0.05). Cox proportional hazards regression model showed that miR-107 may be an independent factor affecting the prognosis of colorectal cancer patients (P < 0.05). The hazard ratio (HR) was 5.165. MiR-107 is highly expressed in colorectal cancer tissues and is closely related to the pathogenesis, progression, and metastasis of colorectal cancer. MiR-107 is expected to become a new molecular marker to assist the diagnosis, treatment effect and prognosis evaluation of colorectal cancer, and may also become a new target for colorectal cancer biotherapy.