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Correlation between the conformation of Escherichia coli -10 hexamer sequences and promoter strength: use of orthophenanthroline cuprous complex as a structural index.

Authors
  • Spassky, A
  • Rimsky, S
  • Buc, H
  • Busby, S
Type
Published Article
Journal
The EMBO journal
Publication Date
Jun 01, 1988
Volume
7
Issue
6
Pages
1871–1879
Identifiers
PMID: 3049079
Source
Medline
License
Unknown

Abstract

The lac and gal control regions contain two functional overlapping promoters P1 and P2. Point mutations can shift transcription from P1 to P2 and vice versa. We show that the reactivity of DNA fragments towards nucleolytic attack with orthophenanthroline cuprous complex can be used to predict which promoter competes more efficiently for RNA polymerase binding. Furthermore, similar changes in reactivity are observed as closed complexes isomerize to form the final open complexes, provided that the functional start is taken as a reference. We found a correlation between the reactivity pattern of -10 regions in uncomplexed DNA and the rate of open complex formation.

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