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Corrected integrated density: a novel method for liver elastic fibers quantification in chronic hepatitis C

Authors
  • Leite, Carine1
  • Starosta, Rodrigo Tzovenos2
  • Trindade, Eduardo Neubarth3
  • Trindade, Manoel Roberto Maciel1, 3
  • Álvares-da-Silva, Mário Reis1, 4
  • Cerski, Carlos Thadeu Schmidt1, 4
  • 1 Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil , Porto Alegre (Brazil)
  • 2 Post-Graduate Program on Genetics and Molecular Biology, UFRGS, Porto Alegre, RS, Brazil , Porto Alegre (Brazil)
  • 3 Hospital de Clínicas de Porto Alegre (HCPA), UFRGS, Porto Alegre, RS, Brazil , Porto Alegre (Brazil)
  • 4 HCPA, UFRGS, Porto Alegre, RS, Brazil , Porto Alegre (Brazil)
Type
Published Article
Journal
Surgical and Experimental Pathology
Publisher
BioMed Central
Publication Date
Feb 15, 2020
Volume
3
Issue
1
Identifiers
DOI: 10.1186/s42047-020-0055-6
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundElastic fibers deposition is triggered during liver fibrosis and is related to worse clinical prognosis in chronic hepatitis C patients. This study aimed to verify if a new method for elastic fiber quantification can be used to discriminate between different degrees of fibrosis in liver biopsies of patients with hepatitis C.MethodsIndividuals presenting with different degrees of fibrosis in liver biopsy were included. Slides of liver samples were stained with orcein with and without prior oxidation. Morphometric analysis was proceeded, and quantification accomplished by corrected integrated density.ResultsTwenty-seven patients, mean age 52 years-old, 59% women, were included. Elastic fibers density was higher in advanced fibrosis patients and there was a positive correlation with Metavir score (Spearman r = 0.609, p < 0.001), as well as with the noninvasive scores Fib-4 (Pearson r = 0.46, p = 0.029) and APRI (r = 0.52, p = 0.01).ConclusionMorphometric analysis by corrected integrated density demonstrates that elastic fibers abundance is higher in advanced stage of fibrosis in patients with hepatitis C.

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