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Core-shell silver nanoparticles for optical labeling of cells.

Authors
  • Dukes, Kyle D1
  • Christensen, Kenneth A1
  • Chumanov, George2
  • 1 Department of Chemistry, Clemson University, Clemson, SC 29634, USA.
  • 2 Department of Chemistry, Clemson University, Clemson, SC 29634, USA. Electronic address: [email protected]
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier
Publication Date
Aug 01, 2014
Volume
458
Pages
43–48
Identifiers
DOI: 10.1016/j.ab.2014.04.015
PMID: 24755004
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Silver nanoparticles have been modified with self-assembled monolayers of hydroxyl-terminated long chain thiols and encapsulated with a silica shell. The resulting core-shell nanoparticles were used as optical labels for cell analysis using flow cytometry and microscopy. The excitation of plasmon resonances in nanoparticles results in strong depolarized scattering of visible light, permitting detection at the single nanoparticle level. The nanoparticles were modified with neutravidin via epoxide-azide coupling chemistry, to which biotinylated antibodies targeting cell surface receptors were bound. The nanoparticle labels exhibited long-term stability in solutions with high salt concentrations without aggregation or silver etching. Labeled cells exhibited two orders of magnitude enhancement of the scattering intensity compared with unlabeled cells. Copyright © 2014 Elsevier Inc. All rights reserved.

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