Affordable Access

deepdyve-link
Publisher Website

Core–shell silver nanoparticles for optical labeling of cellsSilver nanoparticles for optical labeling of cells

Publisher
Elsevier Inc.
Volume
458
Identifiers
DOI: 10.1016/j.ab.2014.04.015
Keywords
Disciplines
  • Chemistry
License
Unknown

Abstract

Abstract Silver nanoparticles have been modified with self-assembled monolayers of hydroxyl-terminated long chain thiols and encapsulated with a silica shell. The resulting core–shell nanoparticles were used as optical labels for cell analysis using flow cytometry and microscopy. The excitation of plasmon resonances in nanoparticles results in strong depolarized scattering of visible light, permitting detection at the single nanoparticle level. The nanoparticles were modified with neutravidin via epoxide–azide coupling chemistry, to which biotinylated antibodies targeting cell surface receptors were bound. The nanoparticle labels exhibited long-term stability in solutions with high salt concentrations without aggregation or silver etching. Labeled cells exhibited two orders of magnitude enhancement of the scattering intensity compared with unlabeled cells.

Report this publication

Statistics

Seen <100 times