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Core needle biopsy wash as a tool for acquiring additional diagnostic material for laboratory testing.

Authors
  • Mojica, Wilfrido1
  • Cwiklinski, Katherine2
  • Jin, Xiaobing3
  • Liu, Weiguo4
  • Yergeau, Donald5
  • 1 Pathology and Anatomical Sciences, University at Buffalo, Buffalo, New York, USA [email protected]
  • 2 Medicine, University at Buffalo, Buffalo, New York, USA.
  • 3 Pathology and Anatomical Sciences, University at Buffalo, Buffalo, New York, USA.
  • 4 Pathology, New York University Medical Center, New York City, New York, USA.
  • 5 NYS Center of Excellence in Bioinformatics and Life Sciences, UB Genomics and Bioinformatics Core, Buffalo, New York, USA.
Type
Published Article
Journal
Journal of Clinical Pathology
Publisher
BMJ
Publication Date
May 01, 2022
Volume
75
Issue
5
Pages
345–349
Identifiers
DOI: 10.1136/jclinpath-2020-207318
PMID: 33649141
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To determine if a simple prewash step added to the processing workflow of tissue procurement by a core needle biopsy device will recover enough cells to expand the laboratory testing armamentarium. Tissue was obtained from unfixed resection specimens using a core needle device and washed in a buffered solution before fixation. This creates a liquid aliquot from which dislodged cells can be kept and separated from the tissue specimen, the latter of which can then undergo traditional formalin-fixed, paraffin-embedded processing. Cells dislodged from the tissue during the biopsy procedure are recoverable, are representative of the tissue section and of sufficient quantities for additional laboratory testing. The core needle biopsy wash is an under-recognised and underutilised approach to extending the diagnostic capabilities of the limited amount of targeted material obtained during this common procedure. The ability to recover supplemental amounts of diagnostic material yields great potential as a substrate for a multitude of current and developing laboratory assays. © Author(s) (or their employer(s)) 2022. No commercial re-use. See rights and permissions. Published by BMJ.

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