Affordable Access

Access to the full text

Core clock regulators in dexamethasone-treated HEK 293T cells at 4 h intervals

Authors
  • Beta, Rafailia A. A.1
  • Arsenopoulou, Zoi V.1
  • Kanoura, Amalia1
  • Dalkidis, Dimitrios1
  • Avraamidou, Rafaela1
  • Balatsos, Nikolaos A. A.1
  • 1 University of Thessaly, Larissa, Greece , Larissa (Greece)
Type
Published Article
Journal
BMC Research Notes
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jan 28, 2022
Volume
15
Issue
1
Identifiers
DOI: 10.1186/s13104-021-05871-7
Source
Springer Nature
Keywords
Disciplines
  • Research Note
License
Green

Abstract

ObjectiveThe study of the circadian clock and its mechanisms is easily facilitated through clock resetting in cell culture. Among the various established synchronizers of the circadian clock in cell culture (temperature, serum shock, glucocorticoids), the artificial glucocorticoid Dexamethasone (DEX) is the most widely used. DEX treatment as a protocol to reset the circadian clock in culture gives simple readout with minimal laboratory requirements. Even though there are many studies regarding clock resetting in culture using DEX, reference points or expression patterns of core clock genes and their protein products are scarce and sometimes contradict other works with similar methodology. We synchronise a cell line of human origin with DEX to be used for studies on circadian rhythms.ResultsWe treat HEK 293T cells with DEX and describe the patterns of mRNA and proteins of core clock regulators, while making a clear point on how CLOCK is less than an ideal molecule to help monitor rhythms in this cell line.

Report this publication

Statistics

Seen <100 times