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Corazonin Signaling Is Required in the Male for Sperm Transfer in the Oriental Fruit Fly Bactrocera dorsalis

Authors
  • Hou, Qiu-Li1, 2
  • Chen, Er-Hu1, 2
  • Jiang, Hong-Bo1, 2
  • Yu, Shuai-Feng1
  • Yang, Pei-Jin1
  • Liu, Xiao-Qiang1, 2
  • Park, Yoonseong3
  • Wang, Jin-Jun1, 2
  • Smagghe, Guy1, 2, 4
  • 1 Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing , (China)
  • 2 Academy of Agricultural Sciences, Southwest University, Chongqing , (China)
  • 3 Department of Entomology, Kansas State University, Manhattan, KS , (United States)
  • 4 Department of Plants and Crops, Ghent University, Ghent , (Belgium)
Type
Published Article
Journal
Frontiers in Physiology
Publisher
Frontiers Media SA
Publication Date
Jun 04, 2018
Volume
9
Identifiers
DOI: 10.3389/fphys.2018.00660
Source
Frontiers
Keywords
Disciplines
  • Physiology
  • Original Research
License
Green

Abstract

Corazonin (Crz) is a widely distributed neuropeptide (or neurohormone) in insects with diverse physiological functions. The present study aimed to reveal the functions of Crz and its receptor (CrzR) in the regulation of sexual behavior and fertility in male Bactrocera dorsalis. Tissue-specific expression analyses showed that the BdCrz transcript was most abundant in the central nervous system (CNS), and the BdCrzR transcript was most abundant in both the fat body and CNS. Immunochemical localization confirmed that three pairs of Crz-immunoreactive neurons are located in the dorsolateral protocerebrum region of male adult brain. Importantly, RNAi-mediated Crz knockdown lengthened mating duration in males, and knockdown of Crz or CrzR strongly decreased male fertility in the following 3 days, while the courtship behavior and mating efficiency were not affected. The reduced number of sperm in the reproductive organs of mated females indicated that Crz knockdown in males reduced sperm transfer. The findings of this study indicate that Crz contributes to the reproductive physiology of the oriental fruit fly B. dorsalis by regulating sperm transfer in male adults.

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