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Copper oxide nanoparticles as an effective anti-biofilm agent against a copper tolerant marine bacterium, Staphylococcus lentus.

Authors
  • Padmavathi, Alwar Ramanujam1
  • Sriyutha Murthy, P1, 2
  • Das, Arindam3, 4
  • Nishad, Padala Abdul1
  • Pandian, Ramanathasamy3
  • Rao, Toleti Subba1, 2
  • 1 Water and Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam, Tamil Nadu, India. , (India)
  • 2 Life Sciences Department, Homi Bhabha National Institute, Mumbai, India. , (India)
  • 3 Surface and Nanoscience Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamil Nadu, India. , (India)
  • 4 Chemical Sciences Department, Homi Bhabha National Institute, Mumbai, India. , (India)
Type
Published Article
Journal
Biofouling
Publisher
Informa UK (Taylor & Francis)
Publication Date
Oct 01, 2019
Volume
35
Issue
9
Pages
1007–1025
Identifiers
DOI: 10.1080/08927014.2019.1687689
PMID: 31718302
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Biofilm formation on antifouling coatings is a serious concern in seawater cooling systems and the maritime industry. A prolific biofilm forming strain (Staphylococcus lentus), possessing high tolerance (>1,000 µg ml-1) to dissolved copper ions (Cu++) was isolated from titanium coupons exposed in the coastal waters of Kalpakkam, east coast of India. S. lentus formed increased biofilm (p < 0.05) at 100 µg ml-1 of Cu++ ions, when compared with the untreated control. To combat biofilm formation of this strain, the efficacy of copper oxide nanoparticles synthesized from copper nitrate by varying the concentrations of hexamine and cetyl trimethyl ammonium bromide (CTAB), was investigated. Complete (100%) inhibition of biofilm formation was observed with plain CuO NP (0.5 M hexamine, uncapped) at 1,000 µg ml-1. Capping with CTAB, influenced the morphology and the purity of the synthesized CuO NPs but did not alter their surface charge. Capping reduced metal ion release from CuO NPs and their antibacterial and anti-biofilm property against S. lentus. Overall, uncapped CuO NPs were effective in controlling biofilm formation of S. lentus. Concurrent release of copper ions and contact mediated physical damage by CuO NPs offer a promising approach to tackle metal tolerant biofilm bacteria.

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