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Cooperative regulation of Gja1 expression by members of the AP-1 family cJun and cFos in TM3 Leydig and TM4 Sertoli cells.

Authors
  • Ghouili, Firas1
  • Martin, Luc J2
  • 1 Biology Department, Université de Moncton, Moncton, New-Brunswick E1A 3E9, Canada. , (Canada)
  • 2 Biology Department, Université de Moncton, Moncton, New-Brunswick E1A 3E9, Canada. Electronic address: [email protected] , (Canada)
Type
Published Article
Journal
Gene
Publication Date
Nov 30, 2017
Volume
635
Pages
24–32
Identifiers
DOI: 10.1016/j.gene.2017.09.017
PMID: 28903063
Source
Medline
Keywords
License
Unknown

Abstract

Within the testis, connexin43 encoded by Gja1 plays an important role in cell-to-cell communication between Leydig cells as well as between Sertoli cells and spermatogonia. In the adult male, Leydig cells are the principal producers of testosterone sustaining spermatogenesis, while Sertoli cells nourish, protect and support the differentiating germ cells. It has been shown previously that members of the AP-1 family regulate Gja1 expression in myometrial cells, suggesting that such regulatory mechanism may also be relevant within the testis. Thus, we performed cotransfections of AP-1 expression plasmids with different mouse Gja1 promoter/luciferase reporter constructs within TM3 Leydig and TM4 Sertoli cells. We showed that a functional cooperation between cJun and cFos activates Gja1 expression and requires an AP-1 DNA regulatory element located between -132 and -26 bp. In addition, such synergy relies on the recruitment of cFos to this region of the mouse Gja1 promoter. Hence, our data indicate that AP-1 members are important for optimal expression of Gja1 within Sertoli and Leydig cells from the testis.

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