Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope.
Neuroscience Unit, Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa, Canada. [email protected]
- Published Article
- Publication Date
Mar 10, 2004
Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/15046170