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Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope.

Authors
  • Ridsdale, Andrew1
  • Micu, Ileana
  • Stys, Peter K
  • 1 Neuroscience Unit, Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa, Canada. [email protected] , (Canada)
Type
Published Article
Journal
Applied optics
Publication Date
Mar 10, 2004
Volume
43
Issue
8
Pages
1669–1675
Identifiers
PMID: 15046170
Source
Medline
License
Unknown

Abstract

Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.

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