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A convenient thiazole orange fluorescence assay for the evaluation of DNA duplex hybridization stability.

Authors
  • Liang, Minmin1
  • Liu, Xinrong
  • Nakamura, Kayoko
  • Chen, Xiangji
  • Cheng, Dengfeng
  • Liu, Guozheng
  • Dou, Shuping
  • Wang, Yi
  • Rusckowski, Mary
  • Hnatowich, Donald J
  • 1 Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Type
Published Article
Journal
Molecular Imaging and Biology
Publisher
Springer-Verlag
Publication Date
Jan 01, 2009
Volume
11
Issue
6
Pages
439–445
Identifiers
DOI: 10.1007/s11307-009-0221-4
PMID: 19444399
Source
Medline
License
Unknown

Abstract

When study duplexes shown to be stable in serum were incubated with their target cells, the assay successfully detected evidence of dissociation, most likely by an antisense mechanism. Thus, a TO fluorescence assay has been developed that is capable of detecting the dissociation of DNA duplexes.

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