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Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions

Authors
  • Co, Julia Y.1
  • Margalef-Català, Mar1
  • Li, Xingnan2
  • Mah, Amanda T.2
  • Kuo, Calvin J.2
  • Monack, Denise M.3
  • Amieva, Manuel R.1, 3
  • 1 Department of Pediatrics, Division of Infectious Diseases, Stanford University, Stanford, CA 94305, USA
  • 2 Department of Medicine, Division of Hematology, Stanford University, Stanford, CA 94305, USA
  • 3 Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Feb 26, 2019
Volume
26
Issue
9
Pages
2509–2520
Identifiers
DOI: 10.1016/j.celrep.2019.01.108
PMID: 30811997
PMCID: PMC6391775
Source
PubMed Central
Keywords
License
Unknown

Abstract

Co et al. describe a method to reverse human enteroid polarity, thus enabling access to the apical epithelium. Upon removing ECM scaffold proteins, basal-out enteroids evert to apical-out polarity in a β1 integrin-dependent manner. Demonstrated applications for this model include evaluating barrier integrity, monitoring nutrient uptake, and examining bacterial infection.

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