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Controlled gating and electrical detection of single 50S ribosomal subunits through a solid-state nanopore in a microfluidic chip.

Authors
  • Mi, Rudenko
  • Mr, Holmes
  • Dn, Ermolenko
  • Ej, Lunt
  • S, Gerhardt
  • Hf, Noller
  • Dw, Deamer
  • A, Hawkins
  • Holger Schmidt
Type
Published Article
Journal
Biosensors and Bioelectronics
Publisher
Elsevier
Volume
29
Issue
1
Pages
34–34
Identifiers
DOI: 10.1016/j.bios.2011.07.047
Source
UCSC Bioengineering biomedical-ucsc
License
Unknown

Abstract

We describe analysis and control of 50S ribosomal subunits by a solid-state 45nm diameter nanopore incorporated in a microfluidic chip. When used as a resistive pulse sensor, translocation of single 50S subunits through the nanopore produces current blockades that have a linear dependence on applied voltage. Introduction of individual subunits into the fluidic channel shows a threshold behavior that allows controlled entry of individual 50S ribosomal subunits. The incorporation of nanopores into a larger optofluidic chip system opens possibilities for electrical and optical studies of single ribosomes in well-defined and rapidly variable chemical environments.

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