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Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells

Authors
  • Shin, Jiyung J.1, 2, 3
  • Schröder, Markus S.3
  • Caiado, Francisco4
  • Wyman, Stacia K.1, 2
  • Bray, Nicolas L.1, 2
  • Bordi, Matteo3
  • Dewitt, Mark A.1, 2
  • Vu, Jonathan T.1, 2
  • Kim, Won-Tae2
  • Hockemeyer, Dirk2
  • Manz, Markus G.4
  • Corn, Jacob E.1, 2, 3
  • 1 Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA
  • 2 Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
  • 3 Department of Biology, ETH Zürich, 8093 Zürich, Switzerland
  • 4 Department of Medical Oncology and Hematology, University Hospital Zurich and University of Zurich, 8091 Zurich, Switzerland
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Sep 01, 2020
Volume
32
Issue
9
Identifiers
DOI: 10.1016/j.celrep.2020.108093
PMID: 32877675
PMCID: PMC7487781
Source
PubMed Central
Keywords
License
Unknown

Abstract

Shin et al. find that quiescent blood stem cells only perform error-prone NHEJ to repair double-strand breaks, whereas cycling blood stem cells perform both error-prone NHEJ and precise HDR. They show that inducing quiescence after a short period of cycling yields blood stem cells harboring high levels of HDR.

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