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Contrasting of biological samples for X-ray synchrotron microtomography.

Authors
  • Efimova, O I1
  • Khlebnikov, A S
  • Senin, R A
  • Voronin, P A
  • Anokhin, K V
  • 1 Laboratory of Neurobiology of Memory, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences; Neurocognitive NTK, Kurchatov NBIC-center, National Research Centre "Kurchatov Institute"; Synchrotron and Neutron NTK, Kurchatov NBIC-center, National Research Centre "Kurchatov Institute", Moscow, Russia. [email protected]
Type
Published Article
Journal
Bulletin of experimental biology and medicine
Publication Date
Aug 01, 2013
Volume
155
Issue
4
Pages
413–416
Identifiers
PMID: 24143358
Source
Medline
License
Unknown

Abstract

The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed by a 48-h washout in phosphate buffered saline had maximum contrast and lowest compression artifacts. Processing of samples by this protocol allowed detecting zones of active proliferation. Incubation of brain samples for 120 h in 7.6% meglumine/sodium diatrizoate without washout ensured the best contrast during myelin identification.

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