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A contradictory action of procoagulant ficin by a fibrinolytic serine protease from Egyptian Ficus carica latex

Authors
  • Hamed, Mohamed B.1
  • El-Badry, Mohamed O.1
  • Kandil, Eman I.2
  • Borai, Ibrahim H.2
  • Fahmy, Afaf S.1
  • 1 Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokii, Cairo, Egypt
  • 2 Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Type
Published Article
Journal
Biotechnology Reports
Publisher
Elsevier
Publication Date
Jun 20, 2020
Volume
27
Identifiers
DOI: 10.1016/j.btre.2020.e00492
PMID: 32642455
PMCID: PMC7334393
Source
PubMed Central
Keywords
License
Unknown

Abstract

Ficus carica is one of the most popular and edible plants. Its trees emanate latex of high medical importance. The well-studied procoagulant effect of ficin is a hallmark of this latex which protrudes an interesting question of how can the plant control this effect? In the present work, we purified and characterized a serine protease (FPIII) with fibrinolytic activity from F. carica latex and study the anticoagulant character of the latex. FPIII was inhibited by PMSF and its molecular weight was 48 kDa. The optimum pH and temperature of FPIII were detected at 8.5 and 60 °C, respectively. The activation energy of FPIII was 7 kcal/mol and was thermal stable up to 60 °C. FPIII tended to hydrolyze different protein substrates and showed a good catalytic efficiency (Kcat/Km). The anticoagulant effects and fibrinogenolytic activities of latex crude extract and FPIII were detected, which controls the procoagulant effect of ficin.

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