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Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP).

Authors
  • Moon, Hui-Sung1
  • Kwon, Kiho
  • Kim, Seung-Il
  • Han, Hyunju
  • Sohn, Joohyuk
  • Lee, Soohyeon
  • Jung, Hyo-Il
  • 1 School of Mechanical Engineering, Yonsei University, 262 Seongsan-no Seodaemun-gu, Seoul, 120-749, South Korea. , (North Korea)
Type
Published Article
Journal
Lab on a Chip
Publisher
The Royal Society of Chemistry
Publication Date
Mar 21, 2011
Volume
11
Issue
6
Pages
1118–1125
Identifiers
DOI: 10.1039/c0lc00345j
PMID: 21298159
Source
Medline
License
Unknown

Abstract

Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer, so their isolations and quantifications are important for biomedical applications such as cancer prognosis and measuring the responses to drug treatments. In this paper, we present the development of a microfluidic device for the separation of CTCs from blood cells based on the physical properties of cells. For use as a CTC model, we successfully separated human breast cancer cells (MCF-7) from a spiked blood cell sample by combining multi-orifice flow fractionation (MOFF) and dielectrophoretic (DEP) cell separation technique. Hydrodynamic separation takes advantage of the massive and high-throughput filtration of blood cells as it can accommodate a very high flow rate. DEP separation plays a role in precise post-processing to enhance the efficiency of the separation. The serial combination of these two different sorting techniques enabled high-speed continuous flow-through separation without labeling. We observed up to a 162-fold increase in MCF-7 cells at a 126 µL min(-1) flow rate. Red and white blood cells were efficiently removed with separation efficiencies of 99.24% and 94.23% respectively. Therefore, we suggest that our system could be used for separation and detection of CTCs from blood cells for biomedical applications.

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