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Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.

Authors
  • Lavine, Jeremy A
  • Raess, Philipp W
  • Davis, Dawn B
  • Rabaglia, Mary E
  • Presley, Brent K
  • Keller, Mark P
  • Beinfeld, Margery C
  • Kopin, Alan S
  • Newgard, Christopher B
  • Attie, Alan D
Type
Published Article
Journal
Molecular Endocrinology
Publisher
The Endocrine Society
Publication Date
Feb 01, 2010
Volume
24
Issue
2
Pages
464–467
Identifiers
DOI: 10.1210/me.2009-0384
PMID: 20081104
Source
Medline
License
Unknown

Abstract

We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1A-expressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets. We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.

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