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Contained metabolic engineering in tomatoes by expression of carotenoid biosynthesis genes from the plastid genome.

Authors
  • Wurbs, David
  • Ruf, Stephanie
  • Bock, Ralph
Type
Published Article
Journal
The Plant journal : for cell and molecular biology
Publication Date
Jan 01, 2007
Volume
49
Issue
2
Pages
276–288
Identifiers
PMID: 17241450
Source
Medline
License
Unknown

Abstract

Applications of chloroplast engineering in agriculture and biotechnology will depend critically on success in extending the crop range of chloroplast transformation, and on the feasibility of expressing transgenes in edible organs (such as tubers and fruits), which often are not green and thus are much less active in chloroplast gene expression. We have improved a recently developed chloroplast-transformation system for tomato plants and applied it to engineering one of the central metabolic pathways in fruits: carotenoid biosynthesis. We report that plastid expression of a bacterial lycopene beta-cyclase gene results in herbicide resistance and triggers conversion of lycopene, the main storage carotenoid of tomatoes, to beta-carotene, resulting in fourfold enhanced pro-vitamin A content of the fruits. Our results demonstrate the feasibility of engineering nutritionally important biochemical pathways in non-green plastids by transformation of the chloroplast genome.

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