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Construction of a genetic map for Caulobacter crescentus.

Authors
  • J T Barrett
  • C S Rhodes
  • D M Ferber
  • B Jenkins
  • S A Kuhl
  • B Ely
Publication Date
Mar 01, 1982
Source
PMC
Keywords
License
Unknown

Abstract

RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid recombinants are recovered at frequencies of 10(-6) and 10(-7) per donor cell. The data from five-factor crosses were subjected to computer-assisted crossover analyses as a rapid method to determine marker order. Using this information and data from additional two- and three-factor crosses mediated by RP4 or the generalized transducing bacteriophage phi Cr30, we constructed the first genetic map for C. crescentus.

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