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Connexin 43 upregulation in burns promotes burn conversion through spread of apoptotic death signals.

Authors
  • Feng, Jiajun1
  • Thangaveloo, Moogaambikai2
  • Ong, Yee Siang3
  • Chong, Si Jack3
  • Joethy, Janna-Vale3
  • Becker, David L4
  • 1 Department of Plastic, Reconstructive & Aesthetic Surgery, Singapore General Hospital, Singapore. Electronic address: [email protected] , (Singapore)
  • 2 Lee Kong Chian School of Medicine, Clinical Sciences Building, Nanyang Technological University 11, Mandalay Road, 308232 Singapore. , (Singapore)
  • 3 Department of Plastic, Reconstructive & Aesthetic Surgery, Singapore General Hospital, Singapore. , (Singapore)
  • 4 Lee Kong Chian School of Medicine, Clinical Sciences Building, Nanyang Technological University 11, Mandalay Road, 308232 Singapore; Skin Research Institute Singapore, Clinical Sciences Building, 11, Mandalay Road, 308232 Singapore. , (Singapore)
Type
Published Article
Journal
Burns : journal of the International Society for Burn Injuries
Publication Date
Sep 01, 2020
Volume
46
Issue
6
Pages
1389–1397
Identifiers
DOI: 10.1016/j.burns.2020.03.011
PMID: 32362363
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Burn wounds continue to worsen after initial injury in a process known as burn conversion, which lasts about 3-5 days. It causes burn wounds to enlarge and deepen, leading to greater morbidity. Apoptosis is one of the factors contributing to the conversion of the zone of stasis into the zone of coagulation. Suppression of apoptosis has been associated with reducing burn conversion. Connexin 43 (Cx43) gap junctions facilitate the spread of apoptotic signals from dying cells to healthy neighbouring cells in injured tissues through the bystander effect. The study is to understand the role of Cx43 in burn conversion. In our study, 15 burn tissue samples were arranged into three groups as early (beginning of burn conversion), intermediate (extensive burn conversion) and late (established burn conversion) burns. We found a striking increase in the amount of Cx43 protein expressed in the dermal fibroblasts (identified with heat shock protein 47 (HSP47) staining) in the zone of stasis in early and intermediate burns. These dermal fibroblasts also express high levels of cleaved-Caspase 3 indicating on-going apoptosis. Our findings suggest that elevation of Cx43 may play an active role in burn conversion spreading apoptosis in the early and intermediate burn wound. Copyright © 2020 Elsevier Ltd and ISBI. All rights reserved.

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