Superposition of the EM reconstructions of p97–p47 and the NSF 20S complex. Beuron F et al. EMBO J. 2006;25:1967-1976 ©2006 by European Molecular Biology Organization Superposition of the EM reconstructions of p97–p47 and the NSF 20S complex. The NSF 20S complex (Furst et al, 2003) was band pass filtered to a resolution of 20 Å. (A) Top view of the superposition of the NSF 20S complex (green) and the p97–p47 AMPPNP map (blue mesh; lacking the antennae). (B) Side view of the superimposed maps. (C) Vertical central section of superimposed maps. The N, D1 and D2 domains of p97 are indicated. Both maps are characterised by a central plug on top of the D1 hexameric ring, which is attributed to α‐SNAP–SNARE densities for the NSF 20S complex and corresponds to the SEP domains of the p47 adaptor in the p97–p47 complex.