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Conformational changes of 4-carboxyglutamic acid-containing protein from bovine bone by binding of alkaline earth ions.

Authors
  • Gundlach, G
  • Voegeli, R
Type
Published Article
Journal
Hoppe-Seyler's Zeitschrift fur physiologische Chemie
Publication Date
Jan 01, 1983
Volume
364
Issue
1
Pages
31–39
Identifiers
PMID: 6601608
Source
Medline
Language
English
License
Unknown

Abstract

The effect of magnesium, calcium, and strontium ions on the bovine bone Gla protein were studied by CD spectroscopy, UV difference spectroscopy and the binding of magnesium and calcium ions was studied by equilibrium dialysis. CD measurements indicate an increase of alpha-helix upon addition of alkaline earth ions. UV difference spectra show a change in the environment of a tyrosine residue. Evaluation of the results of all three studies indicate positive cooperativity in binding of calcium and strontium ions and negative or no cooperativity in binding of magnesium ions.

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