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Confirmation of presumptive Salmonella colonies by the polymerase chain reaction.

Authors
Type
Published Article
Journal
Journal of food protection
Publication Date
Volume
61
Issue
10
Pages
1381–1383
Identifiers
PMID: 9798160
Source
Medline

Abstract

The potential of a genus-specific polymerase chain reaction (PCR) for the confirmation of Salmonella colonies was evaluated on 209 presumptive Salmonella colonies obtained by the standard method ISO 6579. The PCR method employing primers ST11 and ST15 (S. Aabo et al., Mol. Cell. Probes 7:171-178, 1993) gave results identical (100%) to those of the biochemical and serological identification, in terms of discrimination of Salmonella from non-Salmonella strains. PCR could be used directly on the colonies from selective plating media, which allowed a reduction of the time required for confirmation to a maximum of 6 h.

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