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Confirmation that catalase is a glycoprotein.

Authors
  • Pegg, M
  • Crane, D
  • Masters, C
Type
Published Article
Journal
Biochemistry international
Publication Date
Jun 01, 1986
Volume
12
Issue
6
Pages
831–838
Identifiers
PMID: 3017350
Source
Medline
License
Unknown

Abstract

Catalases which had been purified from the livers of mouse, rat and guinea pig were subjected to mild periodate oxidation followed by reduction with sodium boro[3H]hydride in order to test for the presence of sialic acid. A radioactively labelled moiety resulted, which behaved as a derivative of N-acetyl neuraminic acid during mild acid hydrolysis, neuraminidase treatment, ion exchange chromatography and paper chromatography. It is concluded that mammalian catalases are glycoproteins, and possess variable amounts of N-acetyl neuraminic acid in their carbohydrate moiety.

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