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Conductometric monitoring of protein-protein interactions.

Authors
  • Spera, Rosanna
  • Festa, Fernanda
  • Bragazzi, Nicola L
  • Pechkova, Eugenia
  • LaBaer, Joshua
  • Nicolini, Claudio
Type
Published Article
Journal
Journal of Proteome Research
Publisher
American Chemical Society
Publication Date
Dec 06, 2013
Volume
12
Issue
12
Pages
5535–5547
Identifiers
DOI: 10.1021/pr400445v
PMID: 24106799
Source
Medline
License
Unknown

Abstract

Conductometric monitoring of protein-protein and protein-sterol interactions is here proved feasible by coupling quartz crystal microbalance with dissipation monitoring (QCM_D) to nucleic acid programmable protein arrays (NAPPA). The conductance curves measured in NAPPA microarrays printed on quartz surface allowed the identification of binding events between the immobilized proteins and the query. NAPPA allows the immobilization on the quartz surface of a wide range of proteins and can be easily adapted to generate innumerous types of biosensors. Indeed multiple proteins on the same quartz crystal have been tested and envisaged proving the possibility of analyzing the same array for several distinct interactions. Two examples of NAPPA-based conductometer applications with clinical relevance are presented herein, the interaction between the transcription factors Jun and ATF2 and the interaction between Cytochrome P540scc and cholesterol.

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